CRISPR Screen Data for Neutrophil-like HL-60 (PLB-985) Cells
The table below includes data from genome-wide CRISPR screens using differentiated neutrophil-like PLB-985 cells (a sub-line of HL-60), as well as previously measured gene expression data for neutrophil-like PLB-985 cells and primary human neutrophils. More complete gene expression data is available here.
More details about the data and navigating the table are included below.
Reference:
E, Lundgren SM, Kamber RA, Bassik MC, and Collins SR. Parallel CRISPR screens reveal pathways controlling the cell surface levels of the attractant receptor FPR1. (in revison) [Preprint]
CRISPR Screen Data
| wdt_ID | wdt_created_by | wdt_created_at | wdt_last_edited_by | wdt_last_edited_at | Symbol | EnsID | GeneID | Surface FPR1 (Basal) | Surface FPR1 (Stim) | FPR1 Endocytosis | Gene Exp (PLB) | Gene Exp (Neu) | Protein Domains |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | src | 18/02/2026 02:02 PM | src | 18/02/2026 02:02 PM | TSPAN6 | ENSG00000000003 | 7105 | 0.21 | 0.79 | 0.69 | -2.18 | -4.00 | 4HB_MCP_1|Tetraspannin |
| 2 | src | 18/02/2026 02:02 PM | src | 18/02/2026 02:02 PM | TNMD | ENSG00000000005 | 64102 | -1.71 | -1.18 | 0.42 | -4.00 | -4.00 | BRICHOS |
| 3 | src | 18/02/2026 02:02 PM | src | 18/02/2026 02:02 PM | DPM1 | ENSG00000000419 | 8813 | -24.10 | -21.24 | 2.03 | 1.41 | 1.57 | Glyco_tranf_2_2|Glyco_tranf_2_3|Glyco_transf_21|Glycos_transf_2 |
| 4 | src | 18/02/2026 02:02 PM | src | 18/02/2026 02:02 PM | SCYL3 | ENSG00000000457 | 57147 | 1.09 | 1.29 | 0.20 | 0.76 | 0.55 | Pkinase|Pkinase_Tyr |
| 5 | src | 18/02/2026 02:02 PM | src | 18/02/2026 02:02 PM | C1orf112 | ENSG00000000460 | 55732 | 0.99 | 0.55 | -0.96 | 0.40 | 0.26 | DUF4487|Sec23_helical |
| 6 | src | 18/02/2026 02:02 PM | src | 18/02/2026 02:02 PM | FGR | ENSG00000000938 | 2268 | 0.30 | -0.28 | -0.39 | 2.73 | 0.89 | Pkinase|Pkinase_Tyr|SH2|SH3_1|SH3_2|SH3_9 |
| 7 | src | 18/02/2026 02:02 PM | src | 18/02/2026 02:02 PM | CFH | ENSG00000000971 | 3075 | 0.73 | 0.74 | 0.58 | -1.09 | -4.00 | Sushi |
| 8 | src | 18/02/2026 02:02 PM | src | 18/02/2026 02:02 PM | FUCA2 | ENSG00000001036 | 2519 | 0.79 | 0.28 | -0.28 | 0.49 | -2.74 | Alpha_L_fucos|Fucosidase_C |
| 9 | src | 18/02/2026 02:02 PM | src | 18/02/2026 02:02 PM | GCLC | ENSG00000001084 | 2729 | -8.22 | -3.59 | 1.31 | 0.61 | 1.49 | GCS |
| 10 | src | 18/02/2026 02:02 PM | src | 18/02/2026 02:02 PM | NFYA | ENSG00000001167 | 4800 | -0.20 | 2.27 | 2.18 | 1.50 | 1.16 | CBFB_NFYA |
| Symbol | EnsID | GeneID | Surface FPR1 (Basal) | Surface FPR1 (Stim) | FPR1 Endocytosis | Gene Exp (PLB) | Gene Exp (Neu) | Protein Domains |
Screen data
Screen data values are signed Log10 p-value scores.
Surface FPR1 (Basal): Unstimulated cells were separated based on their cell surface levels of the chemoattractant receptor FPR1. Positive values indicated elevated surface FPR1 levels, and negative values indicate reduced surface FPR1 levels. Hits in this screen may reflect phenotypes in FPR1 gene expression, biogenesis, or trafficking to the cell surface.
Surface FPR1 (Stim): Cells were separated based on their surface FPR1 levels after stimulation for 10 minutes with 1 μM fMLF.
FPR1 Endocytosis: An integrated analysis of the two above screens was used to identify target genes for which cell surface FPR1 levels were higher or lower after stimulation than would be expected based on the surface levels in unstimulated cells. Positive values indicate higher than expected FPR1 levels (consistent with a defect in endocytosis or enhanced recycling). Negative values indicate lower than expected FPR1 levels (consistent with a defect in recycling or enhanced endocytosis).
Navigating the data
The gene symbols and Ensembl IDs have clickable links that direct to the corresponding NCBI or Ensembl Gene pages. Some NCBI links are missing due a lack of complete mapping of gene IDs. The gene expression and protein domain data is also missing for these genes.
Search: Data may be searched by symbol, Gene ID or protein domain and sorted by any column.
Expression Values: Expression values are FPKM normalized on a log10 scale. Transcripts that were undetected were assigned an arbitrary value of -4.00. In general, negative values correspond to low transcript levels. Values above zero correspond to genes that are expressed at varying levels, and values above two correspond to highly expressed genes. As the values are on a log10 scale, a difference of 1 for the same gene between two samples corresponds to a 10-fold difference in expression at the transcript level.