Expression data is for primary human and murine neutrophils and PLB985 or HL60 cells before and after differentiation with either 1.3% DMSO or 1.3% DMSO + 0.5% Nutridoma-CS (Sigma). Expression values are FPKM normalized on a log10 scale. Transcripts that were undetected were assigned an arbitrary value of -4.00. Data for PLB985 and HL60 cells is our own RNA-Seq data. Raw data for primary human and mouse neutrophils was collated from previously published studies and analyzed along-side our own1-6. For convenience, links to Dictyostelium discoideum homologs are also displayed. .
For more information, see:
Rincon E, Rocha-Gregg BL, and Collins SR. A map of gene expression in neutrophil-like cell lines. 2018. BMC Genomics [Full text] [PubMed]
Data may be searched by symbol, Gene ID or protein domain and sorted by any column. Users can choose to display a subset of columns by selecting columns from the upper right hand side of the database. Use the icons to print or export the data as desired.
Gene Expression in Neutrophil-like Cell Lines
Symbol | GeneID | Primary Human | PLB985 Undiff | PLB985 +DMSO | PLB985 +Nutri | HL60 Undiff | HL60 +DMSO | HL60 +Nutri | Mouse_Homolog | Mouse | Dicty_Homolog | Prot_Domain |
---|---|---|---|---|---|---|---|---|---|---|---|---|
TSPAN6 | 7105 | -2.18 | -4.00 | -4.00 | -4.00 | -4.00 | -4.00 | -4.00 | Tspan6 | -0.91 | - | 4HB_MCP_1|Tetraspannin |
TNMD | 64102 | -4.00 | -4.00 | -4.00 | -4.00 | -4.00 | -4.00 | -4.00 | Tnmd | -4.00 | - | BRICHOS |
DPM1 | 8813 | 1.41 | 1.72 | 1.58 | 1.57 | 1.75 | 1.55 | 1.60 | Dpm1 | 0.75 | dgtB | Glyco_tranf_2_2|Glyco_tranf_2_3|Glyco_transf_21|Glycos_transf_2 |
SCYL3 | 57147 | 0.76 | 0.34 | 0.54 | 0.55 | 0.25 | 0.45 | 0.40 | Scyl3 | 1.02 | - | Pkinase|Pkinase_Tyr |
C1orf112 | 55732 | 0.40 | 0.97 | 0.18 | 0.26 | 0.77 | 0.03 | 0.20 | BC055324 | 0.28 | - | DUF4487|Sec23_helical |
FGR | 2268 | 2.73 | 0.02 | 0.51 | 0.89 | 0.79 | 1.44 | 1.34 | Fgr | 2.31 | - | Pkinase|Pkinase_Tyr|SH2|SH3_1|SH3_2|SH3_9 |
CFH | 3075 | -1.09 | -4.00 | -4.00 | -4.00 | -4.00 | -4.00 | -2.20 | Cfh | 0.07 | - | Sushi |
FUCA2 | 2519 | 0.49 | -2.54 | -4.00 | -2.74 | 0.57 | 0.52 | 0.85 | Fuca2 | 1.71 | - | Alpha_L_fucos|Fucosidase_C |
GCLC | 2729 | 0.61 | 1.51 | 1.63 | 1.49 | 1.46 | 1.64 | 1.70 | Gclc | 0.25 | gcsA | GCS |
NFYA | 4800 | 1.50 | 1.05 | 1.09 | 1.16 | 0.93 | 0.98 | 1.14 | Nfya | 1.23 | - | CBFB_NFYA |
Symbol | GeneID | Primary Human | PLB985 Undiff | PLB985 +DMSO | PLB985 +Nutri | HL60 Undiff | HL60 +DMSO | HL60 +Nutri | Mouse_Homolog | Mouse | Dicty_Homolog | Prot_Domain |
- Jiang K, Zhu L, Buck MJ, Chen Y, Carrier B, Liu T, et al. Disease-Associated Single-Nucleotide Polymorphisms From Noncoding Regions in Juvenile Idiopathic Arthritis Are Located Within or Adjacent to Functional Genomic Elements of Human Neutrophils and CD4+ T Cells. Arthritis Rheumatol. [Internet]. 2015 [cited 2017 Jul 3];67:1966–77. Available from: http://www.ncbi.nlm.nih.gov/pubmed/25833190
- Wright HL, Thomas HB, Moots RJ, Edwards SW. RNA-seq reveals activation of both common and cytokine-specific pathways following neutrophil priming. PLoS One [Internet]. 2013 [cited 2015 Sep 16];8:e58598. Available from: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=3590155&tool=pmcentrez&rendertype=abstract
- Thomas HB, Moots RJ, Edwards SW, Wright HL. Whose gene is it anyway? the effect of preparation purity on neutrophil transcriptome studies. PLoS One. 2015;10:1–15.
- Cusanovich DA, Daza R, Adey A, Pliner HA, Christiansen L, Gunderson KL, et al. Multiplex single-cell profiling of chromatin accessibility by combinatorial cellular indexing. Science (80-. ). [Internet]. 2015 [cited 2017 Jul 3];348:910–4. Available from: http://www.ncbi.nlm.nih.gov/pubmed/25953818
- Taylor A, Tang W, Bruscia EM, Zhang PX, Lin A, Gaines P, et al. SRF is required for neutrophil migration in response to inflammation. Blood. 2014;123:3027–36.
- Coffelt SB, Kersten K, Doornebal CW, Weiden J, Vrijland K, Hau C-S, et al. IL-17-producing γδ T cells and neutrophils conspire to promote breast cancer metastasis. Nature. 2015;522:345–8.