Gene Expression in Neutrophil-like Cell Lines

Expression data is for primary human and murine neutrophils and PLB985 or HL60 cells before and after differentiation with either 1.3% DMSO or 1.3% DMSO + 0.5% Nutridoma-CS (Sigma). Expression values are FPKM normalized on a log10 scale. Transcripts that were undetected were assigned an arbitrary value of -4.00. Data for PLB985 and HL60 cells is our own RNA-Seq data. Raw data for primary human and mouse neutrophils was collated from previously published studies and analyzed along-side our own1-6. For convenience, links to Dictyostelium discoideum homologs are also displayed. . 

For more information, see:
Rincon E, Rocha-Gregg BL, and Collins SR. A map of gene expression in neutrophil-like cell lines. 2018. BMC Genomics  [Full text] [PubMed]

Data may be searched by symbol, Gene ID or protein domain and sorted by any column. Users can choose to display a subset of columns by selecting columns from the upper right hand side of the database. Use the icons to print or export the data as desired.

Gene Expression in Neutrophil-like Cell Lines

Symbol GeneID Primary Human PLB985 Undiff PLB985 +DMSO PLB985 +Nutri HL60 Undiff HL60 +DMSO HL60 +Nutri Mouse_Homolog Mouse Dicty_Homolog Prot_Domain
TSPAN6 7105 -2.18 -4.00 -4.00 -4.00 -4.00 -4.00 -4.00 Tspan6 -0.91 - 4HB_MCP_1|Tetraspannin
TNMD 64102 -4.00 -4.00 -4.00 -4.00 -4.00 -4.00 -4.00 Tnmd -4.00 - BRICHOS
DPM1 8813 1.41 1.72 1.58 1.57 1.75 1.55 1.60 Dpm1 0.75 dgtB Glyco_tranf_2_2|Glyco_tranf_2_3|Glyco_transf_21|Glycos_transf_2
SCYL3 57147 0.76 0.34 0.54 0.55 0.25 0.45 0.40 Scyl3 1.02 - Pkinase|Pkinase_Tyr
C1orf112 55732 0.40 0.97 0.18 0.26 0.77 0.03 0.20 BC055324 0.28 - DUF4487|Sec23_helical
FGR 2268 2.73 0.02 0.51 0.89 0.79 1.44 1.34 Fgr 2.31 - Pkinase|Pkinase_Tyr|SH2|SH3_1|SH3_2|SH3_9
CFH 3075 -1.09 -4.00 -4.00 -4.00 -4.00 -4.00 -2.20 Cfh 0.07 - Sushi
FUCA2 2519 0.49 -2.54 -4.00 -2.74 0.57 0.52 0.85 Fuca2 1.71 - Alpha_L_fucos|Fucosidase_C
GCLC 2729 0.61 1.51 1.63 1.49 1.46 1.64 1.70 Gclc 0.25 gcsA GCS
NFYA 4800 1.50 1.05 1.09 1.16 0.93 0.98 1.14 Nfya 1.23 - CBFB_NFYA
Symbol GeneID Primary Human PLB985 Undiff PLB985 +DMSO PLB985 +Nutri HL60 Undiff HL60 +DMSO HL60 +Nutri Mouse_Homolog Mouse Dicty_Homolog Prot_Domain
  1. Jiang K, Zhu L, Buck MJ, Chen Y, Carrier B, Liu T, et al. Disease-Associated Single-Nucleotide Polymorphisms From Noncoding Regions in Juvenile Idiopathic Arthritis Are Located Within or Adjacent to Functional Genomic Elements of Human Neutrophils and CD4+ T Cells. Arthritis Rheumatol. [Internet]. 2015 [cited 2017 Jul 3];67:1966–77. Available from: http://www.ncbi.nlm.nih.gov/pubmed/25833190
  2. Wright HL, Thomas HB, Moots RJ, Edwards SW. RNA-seq reveals activation of both common and cytokine-specific pathways following neutrophil priming. PLoS One [Internet]. 2013 [cited 2015 Sep 16];8:e58598. Available from: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=3590155&tool=pmcentrez&rendertype=abstract
  3. Thomas HB, Moots RJ, Edwards SW, Wright HL. Whose gene is it anyway? the effect of preparation purity on neutrophil transcriptome studies. PLoS One. 2015;10:1–15.
  4. Cusanovich DA, Daza R, Adey A, Pliner HA, Christiansen L, Gunderson KL, et al. Multiplex single-cell profiling of chromatin accessibility by combinatorial cellular indexing. Science (80-. ). [Internet]. 2015 [cited 2017 Jul 3];348:910–4. Available from: http://www.ncbi.nlm.nih.gov/pubmed/25953818
  5. Taylor A, Tang W, Bruscia EM, Zhang PX, Lin A, Gaines P, et al. SRF is required for neutrophil migration in response to inflammation. Blood. 2014;123:3027–36.
  6. Coffelt SB, Kersten K, Doornebal CW, Weiden J, Vrijland K, Hau C-S, et al. IL-17-producing γδ T cells and neutrophils conspire to promote breast cancer metastasis. Nature. 2015;522:345–8.