Gene Expression in Neutrophil-like Cell Lines

Expression data is for primary human and murine neutrophils and PLB985 or HL60 cells before and after differentiation with either 1.3% DMSO or 1.3% DMSO + 0.5% Nutridoma-CS (Sigma). Data for PLB985 and HL60 cells is our own RNA-Seq data. Raw data for primary human and mouse neutrophils was collated from previously published studies and analyzed along-side our own1-6. For convenience, links to Dictyostelium discoideum homologs are also displayed. 


Rincon E, Rocha-Gregg BL, and Collins SR. A map of gene expression in neutrophil-like cell lines. 2018. BMC Genomics  [Full text] [PubMed]

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The gene symbols have clickable links that direct to the corresponding NCBI Gene page.

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Expression Values: Expression values are FPKM normalized on a log10 scale. Transcripts that were undetected were assigned an arbitrary value of -4.00. In general, negative values correspond to low transcript levels. Values above zero correspond to genes that are expressed at varying levels, and values above two correspond to highly expressed genes. As the values are on a log10 scale, a difference of 1 for the same gene between two samples corresponds to a 10-fold difference in expression at the transcript level.

Gene Expression in Neutrophil-like Cell Lines

Symbol GeneID Primary Human PLB985 Undiff PLB985 +DMSO PLB985 +Nutri HL60 Undiff HL60 +DMSO HL60 +Nutri Mouse_Homolog Mouse Dicty_Homolog Prot_Domain
TSPAN6 7105 -2.18 -4.00 -4.00 -4.00 -4.00 -4.00 -4.00 Tspan6 -0.91 - 4HB_MCP_1|Tetraspannin
TNMD 64102 -4.00 -4.00 -4.00 -4.00 -4.00 -4.00 -4.00 Tnmd -4.00 - BRICHOS
DPM1 8813 1.41 1.72 1.58 1.57 1.75 1.55 1.60 Dpm1 0.75 dgtB Glyco_tranf_2_2|Glyco_tranf_2_3|Glyco_transf_21|Glycos_transf_2
SCYL3 57147 0.76 0.34 0.54 0.55 0.25 0.45 0.40 Scyl3 1.02 - Pkinase|Pkinase_Tyr
C1orf112 55732 0.40 0.97 0.18 0.26 0.77 0.03 0.20 BC055324 0.28 - DUF4487|Sec23_helical
FGR 2268 2.73 0.02 0.51 0.89 0.79 1.44 1.34 Fgr 2.31 - Pkinase|Pkinase_Tyr|SH2|SH3_1|SH3_2|SH3_9
CFH 3075 -1.09 -4.00 -4.00 -4.00 -4.00 -4.00 -2.20 Cfh 0.07 - Sushi
FUCA2 2519 0.49 -2.54 -4.00 -2.74 0.57 0.52 0.85 Fuca2 1.71 - Alpha_L_fucos|Fucosidase_C
GCLC 2729 0.61 1.51 1.63 1.49 1.46 1.64 1.70 Gclc 0.25 gcsA GCS
NFYA 4800 1.50 1.05 1.09 1.16 0.93 0.98 1.14 Nfya 1.23 - CBFB_NFYA
Symbol GeneID Primary Human PLB985 Undiff PLB985 +DMSO PLB985 +Nutri HL60 Undiff HL60 +DMSO HL60 +Nutri Mouse_Homolog Mouse Dicty_Homolog Prot_Domain
  1. Jiang K, Zhu L, Buck MJ, Chen Y, Carrier B, Liu T, et al. Disease-Associated Single-Nucleotide Polymorphisms From Noncoding Regions in Juvenile Idiopathic Arthritis Are Located Within or Adjacent to Functional Genomic Elements of Human Neutrophils and CD4+ T Cells. Arthritis Rheumatol. [Internet]. 2015 [cited 2017 Jul 3];67:1966–77. Available from:
  2. Wright HL, Thomas HB, Moots RJ, Edwards SW. RNA-seq reveals activation of both common and cytokine-specific pathways following neutrophil priming. PLoS One [Internet]. 2013 [cited 2015 Sep 16];8:e58598. Available from:
  3. Thomas HB, Moots RJ, Edwards SW, Wright HL. Whose gene is it anyway? the effect of preparation purity on neutrophil transcriptome studies. PLoS One. 2015;10:1–15.
  4. Cusanovich DA, Daza R, Adey A, Pliner HA, Christiansen L, Gunderson KL, et al. Multiplex single-cell profiling of chromatin accessibility by combinatorial cellular indexing. Science (80-. ). [Internet]. 2015 [cited 2017 Jul 3];348:910–4. Available from:
  5. Taylor A, Tang W, Bruscia EM, Zhang PX, Lin A, Gaines P, et al. SRF is required for neutrophil migration in response to inflammation. Blood. 2014;123:3027–36.
  6. Coffelt SB, Kersten K, Doornebal CW, Weiden J, Vrijland K, Hau C-S, et al. IL-17-producing γδ T cells and neutrophils conspire to promote breast cancer metastasis. Nature. 2015;522:345–8.