UC Davis Department of Microbiology & Molecular Genetics

Dynamic signaling responses to changing directional cues: Cdc42 signaling activity is monitored as the direction of a chemoattractant gradient is switched.

Genetic interaction maps: quantitative genetic interaction patterns were used to identify new components and connections in the yeast histone H3 K56 acetylation pathway. We are extending these strategies to study signal transduction in mammalian cells.

Analysis of signaling activities in space and time: a psuedocolored image of the activity of Cdc42 in a moving leukocyte is shown. The cell periphery was parametrized into small windows to compare local Cdc42 activity with cell edge protrusion dynamics.

Automated analysis of chemotaxis: we use automated uncaging of chemoattractants and live-cell imaging to measure dynamic chemotactic responses under standardized conditions. Shown are the tracks of differentiated PLB-985 leukocytes responding to a radial attractant gradient.